We are developing a system to follow biofilm formation, under conditions of continuous nutrient efflux, using Single Plane Illumination Microscopy. This type of microscopy offers increased spatial and temporal resolution in addition to low phototoxicity and photobleaching, and has been previously applied to other organisms such as zebrafish and C. elegans. It is of our interest, to apply it to bacteria in order to follow the process of biofilm formation and obtain a better understanding on how these communities develop.